干细胞神经细胞分化的应用

题目:干细胞神经细胞分化的应用

主讲人:洪艺玲 教授

     Western Universtiy of Health Sciences, USA

时间:2017年7月5日  10:00-12:00 am

地点:医学院成义楼105会议室

主讲人简介:

  洪艺玲教授是细胞生物化学博士。长期从事干细胞方面的研究,近年来主要研究干细胞分化成为神经元方面的应用,并在PNAS, Stem cell resarch and Neurotoxicology 等杂志上发表高水平论文。

内容简介:

  人胚胎干细胞具有自我复制更新和分化成为内胚层、外胚层和中胚层细胞的功能。现在有很多种不同的方法来诱导胚胎干细胞分化成为神经元,但是不同的研究中神经元的功能成熟过程有很大差异。我们研究出快速神经分化的模型,进行疾病模拟研究和纳米银颗粒暴露机制研究与毒理机制研究。

Abstract

Stem cell-based neuronal differentiation has provided a unique opportunity for disease modeling toxicity screening and regenerative medicine.Patient-specific induced pluripotent stem cells (hiPSC) can be a powerful tool to unlock the underlying causes of chronic neurodegenerative diseases as well as their treatment.  We have developed a novel defined culture condition and method for generating region-specific neurons and astrocyte network and 3D culture laminated cerebral cortex-like structure, named human cortical spheroids (hCSs) from human embryonic and induced pluripotent stem cells without any genetic manipulation.These 2 D and 3D cultures should allow for a detailed interrogation of human cortical development, function, and disease, and they may prove a versatile platform for generating other neuronal and glial subtypes in vitro. Building off our previously established findings, we are interested in studying the Western Pacific Parkinsonism-Dementia Complex (PDC).  PDC is a largely sporadic neurodegenerative disease with clinical and neuropathological relationships with Parkinson’s disease and Alzheimer’s disease among Chamorros on Guam. This disorder has been linked etiologically to neurotoxic substances and/or genetic factors, including exposure to naturally occurring chemicals in water (certain metals) and/or in a plant (cycad) used for food.  Furthermore,we also the use neurons and astrocytes network derived from human embryonic stem cells (hESCs) as a cellular model to study Silver nanoparticles (AgNPs) induced neurotoxicity.